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KMID : 1149220220300010001
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Korean Journal of Oriental Medical Prescription
2022 Volume.30 No. 1 p.1 ~ p.9
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Regulation of Hippo-YAP AXIS and CYP450 enzymes by herbal pharmaceuticals, Ojeok-san
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Bae Su-Jin
Yun Un-Jung
Bak Seon-Been
Song Yu-Rim
Kim Choon-Ok
Kang Hyung-Won
Kim Young-Woo
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Abstract
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Objectives : This study investigated the protective effect of Ojeok-san (OJS) on cellular damage induced by oxidative stress and whether it induces changes in CYP450 expression.
Methods : To investigate the protective effect, we used cells stimulated by oxidative stress caused by the combination treatment of AA+iron. Changes in CYP450 expression were detected by immunoblotting analysis using Huh7 cells.
Results : We observed that OJS altered the expression of CYP1A2, CYP3A4, CYP2C19, CYP2D6, and CYP2E1. OJS increased cell viability against AA+iron-induced oxidative stress and inhibited mitochondrial dysfunction. OJS increased phosphorylation of LKB1, phosphorylation of AMPK, and phosphorylation of ACC, which are related to the LKB1-AMPK pathway. In addition, phosphorylation of LATS1 and phosphorylation of YAP, which are related to the Hippo-YAP pathway, were increased.
Conclusions : Our results show that OJS has 1) the ability to protect hepatocytes against oxidative stress, and 2) the potential to induce changes in CYP450.
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KEYWORD
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Ojeok-san, Dementia, Neuropsychiatric disorders, P450, AMPK
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